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transcriptome analysis console (tac 4.0.1) software  (Thermo Fisher)


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    Thermo Fisher transcriptome analysis console (tac 4.0.1) software
    Comparative analysis of PAMCELL and ultralow attachment (ULA) methods for DP spheroid formation. (A) Flow cytometry analysis of DP spheroids cultured on PAMCELL and ULA plates showing expression levels of α-SMA, versican, VEGF, fibronectin, PDGFR-α, and PDGFR-β. (B) Circos plot illustrating <t>gene</t> <t>expression</t> <t>profiles</t> and their correlation between 2-dimensional (2D) and 3D culture platforms. (C) Principal component analysis (PCA) of gene expression profiles showing distinct clustering based on culture conditions. (D) Heatmap of differentially expressed genes in DP spheroids cultured on PAMCELL and ULA plates over days 2 and 3. (E) Gene Ontology (GO) analysis highlighting biological processes enriched in DP spheroids cultured in 3D environments. (F) Network map showing biological processes and pathways significantly enriched in 3D cultured DP spheroids, including extracellular matrix (ECM) organization and hair follicle development. FITC, fluorescein isothiocyanate; PE, phycoerythrin; FSC-H, forward scatter height; VarExp, variance explained; p38MAPK, p38 mitogen-activated protein kinases.
    Transcriptome Analysis Console (Tac 4.0.1) Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptome+analysis+console+(tac)+4%2E0%2E1/pmc11675628-101-6-12
    Average 90 stars, based on 1 article reviews
    transcriptome analysis console (tac 4.0.1) software - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "High-Throughput Screening of 3-Dimensional Co-culture Hair Follicle Mimetic Tissue with an Enhanced Extracellular Matrix for the Screening of Hair Growth-Promoting Compounds"

    Article Title: High-Throughput Screening of 3-Dimensional Co-culture Hair Follicle Mimetic Tissue with an Enhanced Extracellular Matrix for the Screening of Hair Growth-Promoting Compounds

    Journal: Biomaterials Research

    doi: 10.34133/bmr.0125

    Comparative analysis of PAMCELL and ultralow attachment (ULA) methods for DP spheroid formation. (A) Flow cytometry analysis of DP spheroids cultured on PAMCELL and ULA plates showing expression levels of α-SMA, versican, VEGF, fibronectin, PDGFR-α, and PDGFR-β. (B) Circos plot illustrating gene expression profiles and their correlation between 2-dimensional (2D) and 3D culture platforms. (C) Principal component analysis (PCA) of gene expression profiles showing distinct clustering based on culture conditions. (D) Heatmap of differentially expressed genes in DP spheroids cultured on PAMCELL and ULA plates over days 2 and 3. (E) Gene Ontology (GO) analysis highlighting biological processes enriched in DP spheroids cultured in 3D environments. (F) Network map showing biological processes and pathways significantly enriched in 3D cultured DP spheroids, including extracellular matrix (ECM) organization and hair follicle development. FITC, fluorescein isothiocyanate; PE, phycoerythrin; FSC-H, forward scatter height; VarExp, variance explained; p38MAPK, p38 mitogen-activated protein kinases.
    Figure Legend Snippet: Comparative analysis of PAMCELL and ultralow attachment (ULA) methods for DP spheroid formation. (A) Flow cytometry analysis of DP spheroids cultured on PAMCELL and ULA plates showing expression levels of α-SMA, versican, VEGF, fibronectin, PDGFR-α, and PDGFR-β. (B) Circos plot illustrating gene expression profiles and their correlation between 2-dimensional (2D) and 3D culture platforms. (C) Principal component analysis (PCA) of gene expression profiles showing distinct clustering based on culture conditions. (D) Heatmap of differentially expressed genes in DP spheroids cultured on PAMCELL and ULA plates over days 2 and 3. (E) Gene Ontology (GO) analysis highlighting biological processes enriched in DP spheroids cultured in 3D environments. (F) Network map showing biological processes and pathways significantly enriched in 3D cultured DP spheroids, including extracellular matrix (ECM) organization and hair follicle development. FITC, fluorescein isothiocyanate; PE, phycoerythrin; FSC-H, forward scatter height; VarExp, variance explained; p38MAPK, p38 mitogen-activated protein kinases.

    Techniques Used: Flow Cytometry, Cell Culture, Expressing

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    Article Snippet: The 83 CEL-files, representing time points 0h (n = 17), 12h (n = 7), 17h (n = 6), 32h (n = 9) and 36h (n = 44), were processed in Transcriptome Analysis Console (TAC 4.0.1, Thermo Fisher Scientific, MA, USA) where data summarization, quantile normalization, gene summaries and statistical analysis were performed in one analysis flow.



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    Comparative analysis of PAMCELL and ultralow attachment (ULA) methods for DP spheroid formation. (A) Flow cytometry analysis of DP spheroids cultured on PAMCELL and ULA plates showing expression levels of α-SMA, versican, VEGF, fibronectin, PDGFR-α, and PDGFR-β. (B) Circos plot illustrating <t>gene</t> <t>expression</t> <t>profiles</t> and their correlation between 2-dimensional (2D) and 3D culture platforms. (C) Principal component analysis (PCA) of gene expression profiles showing distinct clustering based on culture conditions. (D) Heatmap of differentially expressed genes in DP spheroids cultured on PAMCELL and ULA plates over days 2 and 3. (E) Gene Ontology (GO) analysis highlighting biological processes enriched in DP spheroids cultured in 3D environments. (F) Network map showing biological processes and pathways significantly enriched in 3D cultured DP spheroids, including extracellular matrix (ECM) organization and hair follicle development. FITC, fluorescein isothiocyanate; PE, phycoerythrin; FSC-H, forward scatter height; VarExp, variance explained; p38MAPK, p38 mitogen-activated protein kinases.
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    Comparative analysis of PAMCELL and ultralow attachment (ULA) methods for DP spheroid formation. (A) Flow cytometry analysis of DP spheroids cultured on PAMCELL and ULA plates showing expression levels of α-SMA, versican, VEGF, fibronectin, PDGFR-α, and PDGFR-β. (B) Circos plot illustrating <t>gene</t> <t>expression</t> <t>profiles</t> and their correlation between 2-dimensional (2D) and 3D culture platforms. (C) Principal component analysis (PCA) of gene expression profiles showing distinct clustering based on culture conditions. (D) Heatmap of differentially expressed genes in DP spheroids cultured on PAMCELL and ULA plates over days 2 and 3. (E) Gene Ontology (GO) analysis highlighting biological processes enriched in DP spheroids cultured in 3D environments. (F) Network map showing biological processes and pathways significantly enriched in 3D cultured DP spheroids, including extracellular matrix (ECM) organization and hair follicle development. FITC, fluorescein isothiocyanate; PE, phycoerythrin; FSC-H, forward scatter height; VarExp, variance explained; p38MAPK, p38 mitogen-activated protein kinases.
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    Image Search Results


    Comparative analysis of PAMCELL and ultralow attachment (ULA) methods for DP spheroid formation. (A) Flow cytometry analysis of DP spheroids cultured on PAMCELL and ULA plates showing expression levels of α-SMA, versican, VEGF, fibronectin, PDGFR-α, and PDGFR-β. (B) Circos plot illustrating gene expression profiles and their correlation between 2-dimensional (2D) and 3D culture platforms. (C) Principal component analysis (PCA) of gene expression profiles showing distinct clustering based on culture conditions. (D) Heatmap of differentially expressed genes in DP spheroids cultured on PAMCELL and ULA plates over days 2 and 3. (E) Gene Ontology (GO) analysis highlighting biological processes enriched in DP spheroids cultured in 3D environments. (F) Network map showing biological processes and pathways significantly enriched in 3D cultured DP spheroids, including extracellular matrix (ECM) organization and hair follicle development. FITC, fluorescein isothiocyanate; PE, phycoerythrin; FSC-H, forward scatter height; VarExp, variance explained; p38MAPK, p38 mitogen-activated protein kinases.

    Journal: Biomaterials Research

    Article Title: High-Throughput Screening of 3-Dimensional Co-culture Hair Follicle Mimetic Tissue with an Enhanced Extracellular Matrix for the Screening of Hair Growth-Promoting Compounds

    doi: 10.34133/bmr.0125

    Figure Lengend Snippet: Comparative analysis of PAMCELL and ultralow attachment (ULA) methods for DP spheroid formation. (A) Flow cytometry analysis of DP spheroids cultured on PAMCELL and ULA plates showing expression levels of α-SMA, versican, VEGF, fibronectin, PDGFR-α, and PDGFR-β. (B) Circos plot illustrating gene expression profiles and their correlation between 2-dimensional (2D) and 3D culture platforms. (C) Principal component analysis (PCA) of gene expression profiles showing distinct clustering based on culture conditions. (D) Heatmap of differentially expressed genes in DP spheroids cultured on PAMCELL and ULA plates over days 2 and 3. (E) Gene Ontology (GO) analysis highlighting biological processes enriched in DP spheroids cultured in 3D environments. (F) Network map showing biological processes and pathways significantly enriched in 3D cultured DP spheroids, including extracellular matrix (ECM) organization and hair follicle development. FITC, fluorescein isothiocyanate; PE, phycoerythrin; FSC-H, forward scatter height; VarExp, variance explained; p38MAPK, p38 mitogen-activated protein kinases.

    Article Snippet: Acquired data were analyzed using the Transcriptome Analysis Console (TAC 4.0.1) software (Applied Biosystems).

    Techniques: Flow Cytometry, Cell Culture, Expressing

    Depletion of PC decreases oxygen consumption. A , B PCA of gene expression profiles of control (scram) and PC-depleted (shPC25 and shPC28) E0771 cells cultured in low (5.6 mM) glucose DMEM using genes contained in the “electron transport chain” ( A ) and “oxidative phosphorylation” Wikipathways (n = 3/group). C , D Hierarchical clustering of gene expression profiles obtained under conditions described in panels A and B. E – G Basal oxygen consumption rates determined by extracellular flux analysis of the indicated control and PC-depleted cells (n = 3–4/group). Statistical significance calculated using one-way ANOVA with Tukey's post-hoc test ( E , G ) or unpaired student’s t-test ( F )

    Journal: Breast Cancer Research : BCR

    Article Title: Hypoxia-mediated repression of pyruvate carboxylase drives immunosuppression

    doi: 10.1186/s13058-024-01854-1

    Figure Lengend Snippet: Depletion of PC decreases oxygen consumption. A , B PCA of gene expression profiles of control (scram) and PC-depleted (shPC25 and shPC28) E0771 cells cultured in low (5.6 mM) glucose DMEM using genes contained in the “electron transport chain” ( A ) and “oxidative phosphorylation” Wikipathways (n = 3/group). C , D Hierarchical clustering of gene expression profiles obtained under conditions described in panels A and B. E – G Basal oxygen consumption rates determined by extracellular flux analysis of the indicated control and PC-depleted cells (n = 3–4/group). Statistical significance calculated using one-way ANOVA with Tukey's post-hoc test ( E , G ) or unpaired student’s t-test ( F )

    Article Snippet: Quality control and differential gene expression was conducted using Transcriptome Analysis Console (TAC v.4.0.1) software (Affymetrix).

    Techniques: Expressing, Cell Culture